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The BIS-3C Benchtop Shaking Incubator is the high-capacity, advanced-specification model in the BIS Series — engineered for pharmaceutical, biotechnology, and advanced research laboratories that require precise orbital shaking combined with an extended temperature range including active cooling to 4°C. Unlike heating-only shaking incubators, the BIS-3C's refrigeration system enables incubation at temperatures below ambient — opening up cold culture applications, heat-sensitive enzyme assays, and low-temperature fermentation studies that cannot be performed on standard benchtop orbital shaking incubators. With a 450 × 450 mm platform, 33 kg maximum load, 40–300 rpm speed range, 2000 mL flask support, and fully programmable operation, the BIS-3C delivers the throughput, versatility, and precision demanded by advanced life science and pharmaceutical research workflows. Enquire about the BIS-3C today.
AvailableThe BIS-3C Benchtop Shaking Incubator delivers active cooling to 4°C, 40–300 rpm orbital shaking, a 450×450mm high-capacity platform supporting 2000mL flasks, programmable multi-step operation, and touch screen LCD control — providing the extended temperature range, throughput capacity, and operational flexibility required by advanced pharmaceutical, biotechnology, and fermentation research applications.
The defining capability of the BIS-3C is its active refrigeration system, which extends the temperature range from 4°C — the standard refrigeration temperature for biological samples — to 65°C, with 0.1°C control accuracy throughout. This extended range (ambient temperature 20°C assumed) enables applications that are impossible on heating-only shaking incubators: cold room equivalent culture at 4–10°C for cold-adapted microorganisms, enzyme assays requiring temperatures below ambient, low-temperature protein expression protocols (e.g., 16–18°C overnight induction in E. coli for improved soluble protein yield), and temperature ramp studies across the full biological range.
The BIS-3C is operated via a touch screen and LCD control interface providing real-time display and precise digital control of temperature, shaking speed, and timer — all from a single intuitive operator panel. The touch screen interface supports setpoint entry with 0.1°C temperature resolution and ±1 rpm speed accuracy, and provides clear status monitoring during programmable multi-step protocol execution. In advanced research and pharmaceutical environments where protocol documentation and reproducibility are priorities, the digital control interface provides consistent, operator-independent setpoint accuracy across repeated experimental runs.
The BIS-3C extends the shaking speed range to 40–300 rpm — 50 rpm higher than the BIS-2's 250 rpm upper limit. The 300 rpm maximum enables higher-speed aerobic fermentation applications where dissolved oxygen transfer rate is the primary growth-limiting factor, high-agitation enzyme assay conditions, and vigorous mixing protocols for applications such as cell-free expression systems and high-density bacterial culture optimisation. Combined with the 20 mm amplitude, 300 rpm orbital shaking delivers the volumetric mass transfer performance required for advanced fermentation and high-density culture work.
The BIS-3C's 450 × 450 mm platform provides 65% more surface area than the BIS-2's 350 × 350 mm platform — accommodating significantly higher flask numbers per run or larger individual flask formats. The 330 mm interior height provides ample clearance for tall-neck Erlenmeyer flasks at all supported sizes including 2000 mL. Flask capacity: 50 mL × 49, 100 mL × 36, 250 mL × 18, 500 mL × 16, 1000 mL × 9, 2000 mL × 4. The 33 kg maximum load capacity supports fully-loaded large-flask configurations with dense media.
The BIS-3C supports programmable operation — enabling operators to define multi-step protocols with different temperature, speed, and time parameters for each step within a single stored programme. This feature supports advanced research protocols requiring sequential incubation conditions: for example, a bacterial expression protocol combining high-temperature, high-speed growth phase (37°C, 200 rpm, 4 hours) followed by automatic transition to a low-temperature, reduced-speed induction phase (16°C, 150 rpm, 16 hours) — all without operator intervention at the protocol transition point.
The BIS-3C's transparent lid provides continuous visual access to all samples on the 450 × 450 mm platform during shaking and temperature-controlled incubation. In a high-capacity shaking incubator with up to 49 × 50 mL or 4 × 2000 mL flasks running simultaneously, the ability to observe culture turbidity, colour change, foam formation, and flask status without opening the lid — which would disrupt the temperature environment, particularly during sub-ambient cooling protocols — is an important practical advantage for monitoring complex multi-flask experimental runs.
The BIS-3C's support for 2000 mL Erlenmeyer flasks — the largest standard Erlenmeyer format — makes it the appropriate shaking incubator for scale-up culture runs, high-volume fermentation studies, large-batch enzyme production, and bioprocess development work where flask volume directly determines the biological material yield per run. The combination of 2000 mL flask support, 450 × 450 mm platform, and 4°C to 65°C temperature range makes the BIS-3C uniquely versatile for advanced biotechnology research requiring both temperature flexibility and maximum flask volume.
The BIS-3C Benchtop Shaking Incubator integrates a comprehensive safety framework — covering over-temperature protection, vibration-free operation, deviation alarm monitoring, and sustained long-duration reliability — to support safe, unattended operation across complex multi-step, extended fermentation and advanced cell culture protocols.
The BIS-3C's independent over-temperature protection system continuously monitors chamber temperature and automatically cuts heating power if the temperature exceeds the safe upper limit — functioning entirely independently of the primary temperature control and refrigeration circuits. In a high-capacity shaking incubator running advanced biotechnology or pharmaceutical research cultures, an undetected over-temperature event could destroy irreplaceable biological materials representing significant time and resource investment. The independent protection circuit ensures this risk is mitigated even in the event of a primary control system malfunction.
The BIS-3C's high-capacity shaking mechanism is engineered for stable, vibration-free operation at all speeds within the 40–300 rpm range, despite the greater mechanical demands of its larger 450 × 450 mm platform and 33 kg maximum load. Vibration-free performance at high load prevents flask clamp loosening during extended high-speed shaking, protects adjacent sensitive analytical instruments from vibration interference, and ensures that the shaking mechanism maintains consistent orbital motion parameters throughout multi-day fermentation and advanced culture protocols.
The BIS-3C's alarm system activates when operating parameters — temperature, shaking speed, or refrigeration performance — deviate from the programmed setpoints beyond the defined tolerance. In advanced research and pharmaceutical applications where the BIS-3C is used for complex programmed multi-step protocols running unattended, the deviation alarm provides the earliest possible warning of system irregularities — enabling operators to intervene before a deviation compromises an entire culture run or invalidates a time-sensitive experimental protocol that may have been running for hours or days.
The BIS-3C is designed and specified for continuous long-duration operation — including multi-day fermentation runs, 48–72 hour microbial growth studies, extended overnight induction protocols, and long-term cell culture maintenance. The drive mechanism, refrigeration compressor, temperature control electronics, and shaking platform are all specified for sustained duty cycle performance, ensuring that the BIS-3C maintains setpoint accuracy for both temperature and shaking speed throughout extended protocols without mechanical wear or thermal performance degradation.
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